How to count the popular Thoma lamin? by Admin October 16, 2020
Contents
How to count on Thoma slide?The result of counting on the Thoma slide is calculated with the formula A x SF x 10.000. Here, A = number of yeast counted in 16 large squares and SF is the dilution factor. If 10.000, it is a constant used to convert the count result in 0.1 mm3 to the number in 1 ml and obtain a standard result (1 ml = 1 cc = 1 cm3 = 10,000 X 0.1 mm1).
What is the Thoma slide? It works?Thoma slide (hematosimeter) is a method used in microbiology, especially in yeast counting, but it is also used in medicine for sperm and blood counts. Bacteria counting is difficult with this slide.
What is a Neubauer slide?
The improved neubauer slide is called the improved neubauer slide, with triple-drawn margins and finer adjustment of the thoma slide, and this slide is generally used in modern labs. . In order not to double count the cells on the sides while counting the squares, only the cells that touch the left and bottom edges of each square are counted.
How to count the cells?Example1: The sample to be counted in the peritoneal tissue is placed on the Thoma slide, and if the number of cells is very low, the entire area number 1 is counted; For example, let's say 300 cells are counted. The number of cells in the peritoneal fluid = ( 300 / 1 ) X 10.000 = 300 X 10.000 = 3,000,000 cells / ml = 3 x 106 cells / ml.
What is the microscopic count?Microscopic counting methods are based on the principle of examining the microorganisms found in the sample under a microscope. For this purpose, special slides such as Thoma slide and Howard slide have been developed. To be able to use these methods, it is necessary to be a good microscope user.
What does hemocytometer mean?
An instrument used for counting blood cells and spermatozoa, containing a counting chamber with special compartments and two dilution pipettes.
How is the leukocyte count done?Manual leukocyte count: -Venous blood or blood taken from the fingertip is drawn up to the 0.5 mark of the leukocyte pipette. The blood outside the pipette is cleaned. -The Turkish solution is drawn up to the 11 mark of the pipette. -The pipette is taken between the thumb and forefinger and shaken for half a minute.
What is microscopic evaluation?Microscopic examination is a simple, fast and inexpensive method used to diagnose superficial fungal infections (dermatophytes, candida infections and pityriasis versicolor). 1-The patient should be informed about the procedure to be performed. It is not possible to detect fungal structures in the vesicle fluid.
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